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Dedicated imaging systems

Olympus FLIM system

​Contact person: Rinse de Boer

rinse.de.boer@rug.nl

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User fee:

RUG/UMCG: €10,-/h

External use: €100,-/h

Light sources:

  • LIFA multi-LED (Lambert Instruments)

  • Pulsed LED: 405 nm, 474 nm, 550 nm and 630 nm

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Objectives:​​​

  • ​UAPO N 100x/1.49 oil​

  • APO N 60x/1.49 oil

  • PlanApo 60x/1.42 oil

  • UPlan 100x/1.40 oil

  • UplanFL N 60x/1.25 oil

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Filter sets:

  • Excitation BP 420/25     Emission LP 470

  • Excitation BP 475/35     Emission BP 530/43

  • Excitation BP 540/80     Emission BP 647/70 

  • Excitation BP 620/60     Emission BP 700/75

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  • Camera: LIFA vTAU SPAD 300 fps

  • Software: LIFA

  • Environmental chamber, temperature, and CO2 control

Abberior Expert Line (STED)

​Contact person: Rifka Vlijm

r.vlijm@rug.nl

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User fee:

Dependent on type of use

Light sources:

  • Excitation lasers: 405nm (continuous), 488nm (40 MHz), 561nm (40 MHz), 640nm (40 MHz)

  • STED laser: 775nm (40 Mhz, 3W)

  • Adaptive illumination (RESCue & DyMIN) 

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  • Software: Imspector (image acquisition)

  • 2D & 3D STED imaging

  • Four detection channels with RAINBOW detection (400-800nm) and time gating

  • Live-cell compatible (temperature, humidity and CO2 control)

  • Automated imaging

DeltaVision Elite

​Contact person: Rinse de Boer

rinse.de.boer@rug.nl

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User fee:

RUG/UMCG: €10,-/h

External use: €100,-/h

Light sources:

  • SSI solid state illumination

  • 4-laser Photokinetics module, 405, 488, 568 and 640 nm

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Objectives:

  • ​UAPO N 100x/1.49 oil​

  • APO N 60x/1.49 oil

  • PlanApo 60x/1.42 oil

  • UPlan 100x/1.40 oil

  • UplanFL N 60x/1.25 oil

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  • Camera: Pco.edge sCMOS; pixel size 6.5 μm x 6.5 μm, 

  • SoftWoRx 6 acquisition and integrated deconvolution software (on a Linux CentOS 6.3 platform);

  • Localization: PALM/STORM

  • Environmental chamber, temperature, and CO2 control

  • Ultimate focus module

  • ​Automated flexure stage

Olympus TIRF system

​Contact person: Geert van den Boogaart

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User fee:

RUG/UMCG: €10,-/h

External use: €100,-/h

Light sources:

  • Transmittance Thorlabs white LEDepi-fluorescence excitation

  • Thorlabs DC4100 LED source  (405/490/640nm)

  • 488 nm and 561 nm lasers

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Objectives:

  • ​Olympus UAPO 100x/1.49 Oil​

  • ​Olympus UAPO 63x/1.49 Oil

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Filter sets:

  • ​Excitation filter    Quad band filter ZET405/488/561/647x

  • Dichroic mirror    Quad band dichroic ZET405/488/561/647m

  • Emission filter     Quad band filter ZT405/488/561/647rpc

  • Optional: filter wheel with cleaning filters 0/50, ET520/44 , ET605/30

  • OPtional: image splitter: DualView 2 540dclp

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  • Camera: Photometrics prime BSI express sCMOS

  • Software: Micro Manager

  • Environmental chamber, temperature, and CO2 control

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