TEM sample preparation
User fees:
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Chemical fixation + Epon embedding:
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Pre-embedding live cell CLEM:
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Cryo-fixation for morphology:
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Cryo-fixation for immuno EM/CLEM:
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Tokuyasu sectioning (iEM/CLEM):
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Progressive lowering temperature:
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Plunge freezing:
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Negative staining:
​€40,-/sample (>10 = €20,- per sample)
€60,-/sample (>10 = €30,- per sample)
€60,-/sample (>10 = €30,- per sample)
€60,-/sample (>10 = €30,- per sample)
€50,-/sample (>10 = €30,- per sample)
€50,-/sample (>10 = €30,- per sample)
€10,-/grid (In case not internal TEM use)
Included with use TEM
Leica EM ICE High Pressure Freezer
Cryo-fixation of cell suspensions, adhering cells or tissue
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Fixation in 3mm or 6mm carriers (suspensions)
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Fixation in copper tubes for frozen hydrated sectioning and cryo electron microscopy
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Fixations of cells on saphire discs for combining live-cell fluorescence microscopy and electron microscopy
Leica AFS2 + FSP liquid handler
Automated freeze substitution of cryo fixed specimens facilitating analysis of ultra structure at room temperature close to native state
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Freeze substitution in mixture of OsO4 and uranyl in acetone and embedding in epon (morphology)
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Freeze substitution in uranyl acetate in acetone and embedding in lowicryl (iEM/CLEM)
Leica UC6
Room temperture sectioning:
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Ultrathin sectioning for standard 2D TEM analysis
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Semi thin sectioning for electron tomography
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Serial sectioning for array tomography
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Glass knives and diamond knives are availble depending of practical experience
Reichert-Jung Utracut E + FC4
Cryo-ultramicrotomy:
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Tokuyasu cryo-sectioning for immuno electron microscopy
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Tokuyasu cryo-sectioning for Correlative Light and Electron Microscopy (CLEM)
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Diatome Static Line III: Preventing charging of knive and sections
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Glass knives and diamond knives are availble depending of practical experience
Vitrobot
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Contact person: Marc Stuart
Plunge freezing in liquid ethane:
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Cryo-fixation of small particles <500nm imaged directly with cryo electron microscopy​