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Confocal Laser Scanning Microscopes

Zeiss LSM 800

​Contact person: Rinse de Boer

rinse.de.boer@rug.nl

User fee:

RUG/UMCG: €20,-/h

External use: €200,-/h

Light sources (confocal mode):

  • Laser 405 nm​

  • Laser 488 nm

  • Laser 561 nm

  • Laser 640 nm

​

Objectives:

  • Plan apochromat 10x/0.45 DIC II​

  • Plan apochromat 20x/0.8 PH3

  • Plan apochromat 40x/0.95 DIC II

  • Plan apochromat 63x/1.4 DIC II

  • LD PlnN 40x/0.6 DIC II 

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Light sources (Wide field mode):

  • HXP 120V​

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Filter sets: (Wide field mode):

  • Filter set 38          Excitation BP 470/40     Emission BP 525/50

  • Filter set 49          Excitation 365                 Emission BP 445/50

  • Filter set 50          Excitation BP 640/30     Emission BP 690/50

  • Filter set 63 HE    Excitation BP 572/52     Emission BP 629/62 445/50

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  • Software: Zen 2.3

  • Definite focus 1: To eliminate focal drift

  • Heating chamber and objective heating

Zeiss LSM 800 Airyscan

​Contact person: Rinse de Boer

rinse.de.boer@rug.nl

​

User fee:

RUG: €20,-/h

External use: €200,-/h

Light sources:

  • Laser 405 nm​

  • Laser 488 nm

  • Laser 561 nm

  • Laser 640 nm

​

Objectives:

  • Plan apochromat 20x/0.8 DIC II​

  • Plan apochromat 63x/1.4 PH3

  • Plan apochromat 63x/1.4 DIC II

  • Plan apochromat 100x/1.4 DIC II

​

​

  • Array detector (Airyscan 1) 1.7x improved resolution and higher sensitivity​

  • Software: Zen 2.3

  • Definite focus 1: To eliminate focal drift

  • Heating chamber and objective heating

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