Wide field microscopes
Zeiss Observer Z1
User fee:
RUG/UMCG: €10,-/h
External use: €100,-/h
Light sources:
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HXP 120C
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Colibri 1 LED source (365nm,380nm,470nm530nm,555nm
​​​Objectives:
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Plan apochromat 10x/0.25 DIC II​
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Plan apochromat 20x/0.45 DIC II
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Plan apochromat 40x/1.2 DIC III
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Plan apochromat 63x/1.3 DIC II
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Plan apochromat 100x/1.3 DIC II
​​​
Filter sets:
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Filter set 38 Excitation BP 470/40 Emission BP 525/50
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Filter set 43 Excitation BP 545/25 Emission BP 605/70
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Filter set 49 Excitation 365 Emission BP 445/50
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Filter set 50 Excitation BP 640/30 Emission BP 690/50
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Filter set 64 HE Excitation BP 587/25 Emission BP 647/70
​
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Camera: Prime 95B (Photometrics)
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Software: Zen 2.3
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Definite focus 1: To eliminate focal drift
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Heating chamber and objective heating
Zeiss LSM 800
​Contact person: Rinse de Boer
User fee:
RUG/UMCG: €20,-/h
External use: €200,-/h
Light sources (confocal mode):
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Laser 405 nm​
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Laser 488 nm
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Laser 561 nm
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Laser 640 nm
​
Objectives:
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Plan apochromat 10x/0.45 DIC II​
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Plan apochromat 20x/0.8 PH3
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Plan apochromat 40x/0.95 DIC II
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Plan apochromat 63x/1.4 DIC II
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LD PlnN 40x/0.6 DIC II
​
Light sources (Wide field mode):
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HXP 120V​
​
Filter sets: (Wide field mode):
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Filter set 38 Excitation BP 470/40 Emission BP 525/50
-
Filter set 49 Excitation 365 Emission BP 445/50
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Filter set 50 Excitation BP 640/30 Emission BP 690/50
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Filter set 63 HE Excitation BP 572/52 Emission BP 629/62 445/50
​
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Software: Zen 2.3
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Definite focus 1: To eliminate focal drift
-
Heating chamber and objective heating
BioTek Lionhearth FX
User fee:
0-6h: €20,-
6-12h: €40,-
12-24h: €50,-
24-72h: €75,-
>72h: €100,-
​
Light sources:
-
​​
​
Objectives:
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​4x air​
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20x air
​​​​
Filter sets:
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​377/477
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469/525
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586/647
​​​​
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Gen5 4.14 software
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Hardware and software based autofocus
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Temperature and CO2 control
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Live cell imaging
DeltaVision Elite
User fee:
RUG/UMCG: €10,-/h
External use: €100,-/h
Light sources:
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SSI solid state illumination
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4-laser Photokinetics module, 405, 488, 568 and 640 nm
​
Objectives:
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​100x/1.30 oil/phase​
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PlanApo 60x/1.40 oil
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TIRF 100x/1.45 CC340nm​
​​​
​​
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Camera: Pco.edge sCMOS; pixel size 6.5 μm x 6.5 μm,
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SoftWoRx 6 acquisition and integrated deconvolution software (on a Linux CentOS 6.3 platform);
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Localization: PALM/STORM
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Environmental chamber, temperature, and CO2 control
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Ultimate focus module
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​Automated flexure stage
Zeiss Axioscope A1
Light sources:
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HXP 120C
​​​Objectives:
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Plan neofluor 100x/1.3
​​​
Filter sets:
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Filter set 38 Excitation BP 470/40 Emission BP 525/50
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Filter set 20 Excitation BP 546/12 Emission BP 574-640
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Chroma filter Excitation BP 380/30 Emission BP 460/50
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Chroma filter Excitation BP 620/60 Emission BP 700/75
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Filter set 64 HE Excitation BP 587/25 Emission BP 647/70
​
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Camera: CoolSnap
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Software: Micromanager 1.4
User fee:
External use: €50,-/h